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2009, Número 1

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Rev Mex Ing Biomed 2009; 30 (1)


Espectrofluorómetro portátil para realizar mediciones in-vivo e in-situ de material biológico

Moreno GE, Rosa VJM, Fabíla BDA, Pérez GN, Mujica AC, Domíngez-Cherit J

Idioma: Ingles.
Referencias bibliográficas: 28
Paginas: 6-12
Archivo PDF: 250.54 Kb.


RESUMEN

Los métodos de fluorescencia son ampliamente usados en el laboratorio para la medición de concentraciones de especies. Tales mediciones son por tanto no realizadas ni in vivo ni in situ, además de que normalmente son costosas y consumen mucho tiempo debido a los procedimientos de preparación de las muestras. Aquí se presenta un sistema de fluorescencia portátil en base a un mini-espectrómetro, una fuente de luz en base a un led, una fibra óptica bifurcada y una laptop. El espectrofluorómetro es útil para medir in vivo e in situ sustancias fluorescentes tales como tejido humano y otros materiales orgánicos y no orgánicos. El sistema es controlado desde la laptop por medio de un programa escrito en el lenguaje gráfico G de LabVIEW 7.1, el cual mide y procesa los espectros en tiempo real. Se presentan mediciones realizadas con un led de 200 mW que emite a 365 nm.


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