1997, Número 4
Picobirnavirus: nuevo agente viral como probable causante de la diarrea infecciosa aguda en lechones lactantes de granjas porcícolas del estado de Yucatán, México
Puerto SM, Puerto MFI, Valadez GN, González LMR, Polanco MG
Idioma: Español/Inglés
Referencias bibliográficas: 17
Paginas: 309-311
Archivo PDF: 660.59 Kb.
RESUMEN
A new group of RNA virus was described in 1988 by Pereira. This group of viruses has only two segments of RNA; it was called Picobinavirus (PBV). Since then, it has been reported in animals and humans in several countries, but not in Mexico. In this paper the finding of these viruses in Merida, Mexico is reported. Faecal specimens were obtained from two hundred and eighty nine pigs with diarrhoea during their first month of life. Stools were analyzed by polyacrylamide gel electrophoresis (PAGE). Seventy four pigs showed electrophoretic pattern for rotavirus, whereas only three showed a typical electrophoretic pattern for PBV. Characterization of the strains showed segment sizes which ranged from 2600-1600 bp. The nucleic acid was shown double-stranded RNA by nuclease digestion. This pattern resembles PBV. These findings suggest that PBV could be a new group of virus associated with gastroenteritis in pigs.
REFERENCIAS (EN ESTE ARTÍCULO)
Murphy FA, Kings DW. Virus taxonomy. In: Fields BN, Knipe DM, editors. Virology. Vol 1. New York: Raven Press, 1990:9-35.
Pereira HG, Flewett TH, Candeias JAN, Barth OM. A virus with bisegmented double-stranded RNA genome in rat (Oryzomys nigripes) intestines. J Gen Virol 1988;69:2749-2754.
Pereira HG, de Araujo HP, Fialho AM, de Castro L, Monteiro Sp. A virus with bisegmented double stranded RNA genome in guinea pig intestines. Men Inst Oswaldo Cruz 1989;84:137-140.
Pereira HG, Fialho AM, Flewett TH, Teixeira JMS, Andrade ZP. Novel viruses in human faeces. Lancet 1988;9:103-104.
Holmes IH. Rotaviruses. In: Joklik WK, editor. The reoviridae. New York: Plenum, 1983:359-423.
Desselberger U, McCrae M. The rotavirus genome In: Raming RF, editor. Rotaviruses, current topics in microbiology and immunology. Berlin: Springer-Verlag, 1994:31-66.
Ludert JE, Hidalgo M, Gil F, Liprandi F. Identification in porcine faeces of a novel virus with a bisegmented double-stranded RNA genome. Arch Virol 1991;117:97-107.
Gallimore C, Appleton H, Lewis D, Green J Brown D. Detection and characterization of bisegmented double-stranded RNA viruses (picobirnaviruses) in human faecal specimens. J Med Virol 1995;45:135-140.
Alfieri AF, Alfieri AA, Resende JS, Resende NA. A new bisegmented double-stranded RNA virus in avian feces. Arq Bras Med Vet Zoot 1989;40:437-440.
Gallimore C, Green J, Casemore D, Brown D. Detection of a picobirnavirus associated with Cryptosporidium positive stools from humans. Arch Virol 1995;140:1275-1278.
Gallimore C, Lewis D, Brown D. Detection and characterization of a novel bisegmented double-stranded RNA virus (picobirnavirus) from rabbit faeces. Arch Virol 1993;133:63-73.
Gatti MSV, de Pestana AF, Ferraz MMG. Viruses with bisegmented double-stranded RNA in pig faeces. Res vet Sci 1989;47:397-398.
Ludert JE, Liprandi F. Identification of viruses with bi- and trisegmented double-stranded RNA genome in faeces of children with gastroenteritis. Res Virol 1993;144:219-224.
Monteir SP, Pereira HG. Fialho AM, Leite JPG. Viruses with segmented double-stranded RNA in chickens. Arq Bras Med Vet Zoot 1991;42:141-146.
Puerto FI, Polanco G, Puerto M, Ortega G, Góngora R. Diarrea infantil aguda por rotavirus en una población pediátrica de Mérida, Yuc., México. Bol Med Hosp Infant 1989;46:171-174.
Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD. Rapid diagnosis of rotavirus infection by direct detection of viral nucleid acid in silver stained polyacrylamide gels. J Clin Microbiol 1982;16:473-477.
Sambrook J, Fritsch EF, Maniatis T. Nucleases digestion. In: Irwin N, Ford N, Nolan Ch, Ferguson M, Ockler M, editors. Molecular cloning a laboratory manual. New York: Cold Spring Harbor Laboratory Press, 1989:5.83-4,7.75-76.