medigraphic.com
ISSN 1870-199X (Impreso)
Órgano oficial de la Facultad de Odontología, UNAM

2005, Número 1

<< Anterior Siguiente >>

Rev Odont Mex 2005; 9 (1)


Descripción de la microbiota subgingival de sujetos mexicanos con periodontitis crónica

Almaguer-Flores A, Jacobo-Soto V, Sánchez-Vargas LO, Lara-Córdoba M, Alcántara-Maruri E, Ximénez-Fyvie LA

Idioma: Español
Referencias bibliográficas: 52
Paginas: 7-15
Archivo PDF: 310.64 Kb.


RESUMEN

Introducción: Las enfermedades periodontales son infecciones causadas por microorganismos que colonizan la cavidad oral en una estructura compleja llamada biopelícula. Objetivo: Describir la microbiota subgingival de pacientes mexicanos con periodontitis crónica. Metodología: A 56 pacientes (33 con periodontitis crónica y 23 periodontalmente sanos) les fueron tomadas muestras de placa dentobacteriana subgingival. Las muestras fueron evaluadas microbiológicamente utilizando la técnica “chekerboard” para hibridaciones DNA-DNA. Resultados: Todas las especies evaluadas fueron detectadas tanto en sujetos periodontalmente sanos como con periodontitis crónica. El análisis de las muestras de los sujetos de estudio mostró que P. gingivalis (p < 0.001), T. forsythensis (p › 0.01), T. denticola y A. actinomycetemcomitans (p › 0.05) presentaron cuentas promedio significativamente mayores en la población de sujetos con periodontitis crónica. Los pacientes con periodontitis, mostraron además proporciones más elevadas de especies del complejo “rojo” y menores proporciones de especies de Actinomyces y de especies del complejo “amarillo” cuando fueron comparados con pacientes periodontalmente sanos. Conclusiones: Debido a la complejidad de la microbiota subgingival en sujetos mexicanos y a las diferencias con reportes provenientes de otras poblaciones, es necesario profundizar en el conocimiento y entendimiento del papel de las bacterias que intervienen en el proceso de la enfermedad periodontal en nuestro país. De esta manera, podremos ofrecer a los pacientes mexicanos, tratamientos basados en el conocimiento de las características microbiológicas propias de nuestra población.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Albandar J. Global risk factors and risk indicators for periodontal diseases. Periodontol 2000 2002; 29: 177-206.

  2. Albandar J. Periodontal diseases in North America. Periodontol 2000 2002; 29: 31-69.

  3. Ali RW, Johannessen AC, Dahlen G, Socransky SS, Skaug N. Comparison of the subgingival microbiota of periodontally healthy and diseases adults in Northern Cameroon. J Clin Periodontol 1997; 24(11): 830-5.

  4. Baelum V, Scheutz F. Periodontal diseases in Africa. Periodontol 2000 2002; 29 : 79-103.

  5. Choi B, Park S, Yoo Y, Choi S, Chai J, Cho K, Kim C. Detection of major putative periodontopathogens in Korean advanced adult periodontitis patients using a nucleic acid-based approach. J Periodontol 2000; 71(9): 1387-94.

  6. Colombo AP, Teles RP, Torres MC, Souto R, Rosalem WJ, Mendes MC, Uzeda M. Subgingival microbiota of Brazilian subjects with untreated chronic periodontitis. J Periodontol 2002; 73(4): 360-9.

  7. Corbet E, Zee K, Lo E. Periodontal diseases in Asia and Oceania. Periodontol 2000 2002; 29: 122-52.

  8. Costerton J, Cook G, Lamont R. The community architecture of biofilms: dynamic structures and mechanisms. In: Newman HN & Wilson M, ed. Dental Plaque Revisited: Oral Biofilms in Health and Disease. Eastman Dental Institute, University College London 1999: 5-14.

  9. Dahlen G, Manji F, Baelum V, Fejerskov O. Putative periodontopathogens in “diseases” and “non-diseases” persons exhibiting poor oral hygiene. J Clin Periodontol 1992; 19(1): 35-42.

  10. Dahlen G, Wikstrom M. Occurrence of enteric rods, staphylococci and Candida in subgingival samples. Oral Microbiol Immunol 1995; 10(1): 42-6.

  11. Dogan B, Antinheimo J, Cetiner D, Bodur A, Emingil G, Buduneli E, Uygur C, Firatli E, Lakio L, Asikainen S. Subgingival microflora in Turkish patients with periodontitis. J Periodontol 2003; 74(6): 803-14.

  12. Dowsett SA, Kowolik MJ, Archila LA, Eckert GJ, LeBlanc DJ. Subgingival microbiota of indigenous Indians of Central America. J Clin Periodontol 2002; 29(2): 159-67.

  13. Engler-Blum G, Meier M, Frank J, Muller GA. Reduction of background problems in nonradioactive Northern and Southern blot analyses enables higher sensitivity than 32P-based hybridizations. Anal Biochem 1993; 210(2): 235-44.

  14. Ezzo P, Cutler C. Microorganisms as risk indicators for periodontal disease. Periodontol 2000 2003; 32: 24-35.

  15. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132(1): 6-13.

  16. Gjermo P, Rosing C, Susin C, Oppermann R. Periodontal diseases in Central and South America. Periodontol 2000 2002; 29: 70-8.

  17. Haffajee A, Socransky S, Feres M, Ximenez-Fyvie L. Plaque microbiology in health and disease. In: Newman HN & Wilson M, ed. Dental Plaque Revisited: Oral Biofilms in Health and Disease. Eastman Dental Institute, University College London 1999: 255-82.

  18. Haffajee AD, Cugini MA, Dibart S, Smith C, Kent RL, Jr., Socransky SS. Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planing. J Clin Periodontol 1997; 24(10): 767-76.

  19. Haffajee AD, Cugini MA, Tanner A, Pollack RP, Smith C, Kent RL, Jr., Socransky SS. Subgingival microbiota in healthy, well-maintained elder and periodontitis subjects. J Clin Periodontol 1998; 25(5): 346-53.

  20. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 1994; 5: 78-111.

  21. Hamlet SM, Cullinan MP, Westerman B, Lindeman M, Bird PS, Palmer J, Seymour GJ. Distribution of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia in an Australian population. J Clin Periodontol 2001; 28(12): 1163-71.

  22. Kolenbrander P, Andersen R, Clemans D, Whittaker C, Klier C. Potential role of functionally similar coagregation mediators in bacterial succession. In: Newman HN & Wilson M, ed. Dental Plaque Revisited: Oral Biofilms in Health and Disease. Eastman Dental Institute, University College London 1999: 171-86.

  23. Lee J, Choi B, Yoo Y, Choi S, Cho K, Chai J, Kim C. Distribution of periodontal pathogens in Korean aggressive periodontitis. J Periodontol 2003; 74(9): 1329-35.

  24. Leys E, Lyons S, Moeschberger M, Rumpf R, Griffen A. Association of Bacteroides forsythus and a novel Bacteroides phylotype with periodontitis. J Clin Microbiol 2002; 40(3): 821-5.

  25. Listgarten M. Formation of dental plaque and other oral biofilms. In: Newman HN & Wilson M, ed. Dental Plaque Revisited: Oral Biofilms in Health and Disease. Eastman Dental Institute, University College London 1999: 187-210.

  26. Listgarten MA. The structure of dental plaque. Periodontol 2000 1994; 5: 52-65.

  27. Molin S. Microbial activity in biofilm communities. In: Newman HN & Wilson M, ed. Dental Plaque Revisited: Oral Biofilms in Health and Disease. Eastman Dental Institute, University College London 1999: 73-8.

  28. Moore WE, Holdeman LV, Cato EP, Smibert RM, Burmeister JA, Palcanis KG, Ranney RR. Comparative bacteriology of juvenile periodontitis. Infect Immun 1985; 48(2): 507-19.

  29. Moore WE, Holdeman LV, Smibert RM, Hash DE, Burmeister JA, Ranney RR. Bacteriology of severe periodontitis in young adult humans. Infect Immun 1982; 38(3): 1137-48.

  30. Moore WE, Moore LH, Ranney RR, Smibert RM, Burmeister JA, Schenkein HA. The microflora of periodontal sites showing active destructive progression. J Clin Periodontol 1991; 18(10): 729-39.

  31. Moore WE, Moore LV. The bacteria of periodontal diseases. Periodontol 2000 1994; 5: 66-77.

  32. Paster B, Boches S, Galvin J, Ericson R, Lau C, Levanos V, Sahasrabudhe A, Dewhirst F. Bacterial diversity in human subgingival plaque. J Bacteriol 2001; 183(12): 3770-83.

  33. Riviere G, Smith K, Carranza N, Tzagaroulaki E, Kay S, Dock M. Subgingival distribution of Treponema denticola, Treponema socranskii, and pathogen-related oral spirochetes: prevalence and relationship to periodontal status of sampled sites. J Periodontol 1995; 66(10): 829-37.

  34. Sakai Y, Shimauchi H, Ito H, Kitamura M, Okada H. Porphyromonas gingivalis-specific IgG subclass antibody levels as immunological risk indicators of periodontal bone loss. J Clin Periodontol 2001; 28(9): 853-9.

  35. Sela M. Role of Treponema denticola in periodontal diseases. Crit Rev Oral Biol Med 2001; 12(5): 399-413.

  36. Sheiham A, Netuveli G. Periodontal diseases in Europe. Periodontol 2000 2002; 29: 104-21.

  37. Slots J. Bacterial specificity in adult periodontitis. A summary of recent work. J Clin Periodontol 1986; 13(10): 912-7.

  38. Slots J, Rams TE, Feik D, Taveras HD, Gillespie GM. Subgingival microflora of advanced periodontitis in the Dominican Republic. J Periodontol 1991; 62(9): 543-7.

  39. Smith GL, Socransky SS, Smith CM. Rapid method for the purification of DNA from subgingival microorganisms. Oral Microbiol Immunol 1989; 4(1): 47-51.

  40. Socransky S, Haffajee A. Dental biofilms: difficult therapeutic targets. Periodontol 2000 2002; 28: 12-55.

  41. Socransky SS, Haffajee AD. Evidence of bacterial etiology: a historical perspective. Periodontol 2000 1994; 5: 7-25.

  42. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL, Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998; 25(2): 134-44.

  43. Socransky SS, Haffajee AD, Smith C, Dibart S. Relation of counts of microbial species to clinical status at the sampled site. J Clin Periodontol 1991; 18(10): 766-75.

  44. Socransky SS, Smith C, Haffajee AD. Subgingival microbial profiles in refractory periodontal disease. J Clin Periodontol 2002; 29(3): 260-8.

  45. Socransky SS, Smith C, Martin L, Paster BJ, Dewhirst FE, Levin AE. “Checkerboard” DNA-DNA hybridization. Biotechniques 1994; 17(4): 788-92.

  46. Tanner A, Kent R, Maiden MF, Taubman MA. Clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects. J Periodontal Res 1996; 31(3): 195-204.

  47. Tanner A, Maiden MF, Macuch PJ, Murray LL, Kent RL, Jr. Microbiota of health, gingivitis, and initial periodontitis. J Clin Periodontol 1998; 25(2): 85-98.

  48. Timmerman MF, Van der Weijden GA, Arief EM, Armand S, Abbas F, Winkel EG, Van Winkelhoff AJ, Van der Velden U. Untreated periodontal disease in Indonesian adolescents. Subgingival microbiota in relation to experienced progression of periodontitis. J Clin Periodontol 2001; 28(7): 617-27.

  49. Willis S, Smith K, Dunn V, Gapter L, Riviere K, Riviere G. Identification of seven Treponema species in health- and disease-associated dental plaque by nested PCR. J Clin Microbiol 1999; 37(3): 867-9.

  50. Xie H, Cook GS, Costerton JW, Bruce G, Rose TM, Lamont RJ. Intergeneric communication in dental plaque biofilms. J Bacteriol 2000; 182(24): 7067-9.

  51. Ximenez-Fyvie LA, Haffajee AD, Socransky SS. Comparison of the microbiota of supra-and subgingival plaque in health and periodontitis. J Clin Periodontol 2000; 27(9): 648-57.

  52. Zambon J. Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol 1985; 12(1): 1-20.




2020     |     www.medigraphic.com