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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2006, Número 6

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Rev Invest Clin 2006; 58 (6)


Detección de Mycobacterium tuberculosis de muestras respiratorias con el sistema de cultivo líquido MB/BacT y verificado por PCR

García-Elorriaga G, Carrillo-Montes MG, Rey-Pineda G, González-Bonilla CR

Idioma: Ingles.
Referencias bibliográficas: 50
Paginas: 573-579
Archivo PDF: 70.18 Kb.


RESUMEN

Objetivo. Evaluar en el marco clínico el sistema MB/BacT para el aislamiento de Mycobacterium tuberculosis y su verificación mediante PCR. Material y métodos. El estudio incluyó 272 muestras de esputo provenientes de 208 pacientes con el diagnóstico presuntivo de tuberculosis pulmonar. Se realizó la tinción de ZN, así como cultivo en el medio de Löwenstein-Jensen, MB/BacT y PCR. Resultados. Por cultivo en Löwenstein-Jensen resultaron positivas 39 muestras y 42 utilizando el sistema MB/BacT. Los cultivos positivos en el sistema MB/BacT se verificaron con tinción para bacilos ácido alcohol resistentes y por PCR. El desarrollo micobacteriano en MB/BacT se presentó de ocho a 46 días (media 16 días), mientras que en el medio Löwenstein-Jensen el rango fue de 21 a 63 días (media 35 días). Estos resultados demuestran que el sistema semiautomatizado MB/BacT es confiable y más rápido que el método de cultivo manual y puede utilizarse como una buena alternativa para el cultivo primario de Mycobacterium tuberculosis. El ensayo de PCR también permite la identificación exacta y rápida de Mycobacterium tuberculosis directamente del medio líquido positivo.


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