medigraphic.com
ISSN 0001-0944 (Impreso)
Órgano Oficial de la Asociación Dental Mexicana

2008, Número 2

<< Anterior Siguiente >>

Rev ADM 2008; 65 (2)


Daño a la mucosa bucal inducido por fluoruro de sodio en la rata: Aumento en los parámetros apoptóticos

Gutiérrez-Salinas J

Idioma: Español
Referencias bibliográficas: 37
Paginas: 61-68
Archivo PDF: 109.01 Kb.


RESUMEN

Objetivo: Determinar los efectos de la ingesta de fluoruro de sodio (NaF) sobre la mucosa bucal de la rata, manifestado por cambios en la expresión de las proteínas p53, bcl-2 y caspasa-3 como indicadoras de un proceso de apoptosis, así como en la actividad de la enzima amilasa salival como indicador de la función de la mucosa. Material y métodos: ratas macho fueron expuestas a NaF (1 y 50 ppm) hasta por ocho semanas, las cuales fueron sacrificadas semanalmente. La mucosa bucal fue aislada y se determinó la expresión de las proteínas p53, bcl-2 y caspasa-3 por medio de técnicas de Western-blot mientras que la actividad de la enzima amilasa fue determinada por métodos espectrofotométricos. Resultados: mostraron que el tratamiento con 50 ppm de NaF produce un incremento en la expresión de las proteínas p53 y caspasa-3 así como una disminución importante en la actividad de la enzima amilasa. Estos resultados señalan que la intoxicación con NaF puede ser un inductor de apoptosis en la mucosa bucal en ratas intoxicadas hasta por ocho semanas.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Rzeuski R, Chlubek D, Machoy Z. Interaction between fluoride and biological free radical reaction. Fluoride 1998; 31: 43-45.

  2. Mullenix PJ, Denbesten PK, Schunior A, Kernan WJ. Neurotoxicity of sodium fluoride in rats. Neurotoxicolo Theratol 1995; 17: 169-177.

  3. Jacyszyn K, Marut A. Fluoride in blood and urine in humans administered fluoride and exposed to fluoride-polluted air. Fluoride 1986; 19: 26-32.

  4. Monsour PA, Kruger BJ. Effect of fluoride on soft tissues in vertebrates. Fluoride 1985; 18: 53-61.

  5. Goh EH, Neff AW. Effects of fluoride on Xenopus embryo development. Food and Chem Toxicol 2003; 41: 1501-1508.

  6. Machalinska A, Machoy-Mokrzynska A, Marlicz W, Stecewicz I, Machalinski B. NaF-induced apoptosis in human bone marrow and cord blood CD34 positive cells. Fluoride 2001; 34: 258-263.

  7. Holland RI. Fluoride inhibition of protein and DNA synthesis in cells in vitro. Acta Pharmacol Toxicol 1979; 45: 96-101.

  8. Gutiérrez-Salinas J, Morales-González JA. Production of free radicals derived from oxygen and hepatocyte damage. Med Int Mex 2004; 20: 287-295.

  9. Wang AG, Chu QL, He WH, Xia T, Liu JL, Zhang M, Nussier AK, Chen XM, Yang KD. Effects on protein and mRNA expression levels of p53 induced by fluoride in humans embryonic hepatocytes. Toxicol Lett 2005; 158: 158-163.

  10. Refsnes F, Lag T, Skuland T, Samuelsen J, Schwarze P. Fluoride-induced apoptosis and necrosis in a human lung epithelial cell line: involvement of PKA and PKC-mediated mechanism. Toxicol Lett 1998; 95(Supp 1): 107.

  11. Hidaro S, Ando M. Apoptotic cell death following exposure to fluoride in rat alveolar macrophages. Arch Toxicol 1996; 70: 249-251.

  12. Zhan XA, Wang M, Xu ZR, Li WF, Li JX. Evaluation of caspase-dependent apoptosis during fluoride-induced liver lesion in pigs. Arch Toxicol 2005; 78: 145-156.

  13. Morales-González JA, Bueno-Cardoso A, Marichi-Rodriguez F, Gutiérrez-Salinas J. Programmed cell death (apoptosis): the regulating mechanisms of cellular proliferation. Arch Neurocien (Mex) 2004; 9: 85-93.

  14. Darmani H, Al-Hiyasat AS, Elvetieha AM. Effects of sodium fluoride in drinking water on fertility in female mice. Fluoride 2001; 34: 242-249.

  15. Shivarajashankara YM, Shivashankara AR, Gopalakrishna Bhat P, Rao S. Brain lipid peroxidation and antioxidant systems of young rats in chronic fluoride intoxication. Fluoride 2002; 35: 197-203.

  16. Gutiérrez-Salinas J, Garduño-Miranda L, Trejo-Izquierdo E, Díaz-Muñoz M, Vidrio S, Morales-González JA, Hernández-Muñoz R. Redox state and energy metabolism during liver regeneration. Biochem Pharmacol 1999; 58: 1831-1839.

  17. Vaidya MM, Wawant SS, Borges AM, Narres NK, Purandare MC, Bhisey AN. Cytokeratin expression in human fetal tongue and buccal mucosa. J Biosc 2000; 25: 235-242.

  18. Nicolau J, Ferreira ML. In vitro and in vivo effects of fluoride solution on human salivary amylase. Fluoride 2001; 34: 55-60.

  19. Lowry OH, Rosebrough NJ, Farr AI, Randal RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 1: 265-275.

  20. Laemmli VK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.

  21. Towbin H, Gordon J. Immunoblotting and dot immunobindig. Current status and out-look. J Immunol Methods 1984; 72: 313-340.

  22. Gutiérrez-Salinas J, Morales-González JA, Vargas-Castro LD, Marichi-Rodríguez F, Esquivel-Soto J. Changes in laminin expression in periodontal ligament from theet subjet to orthodontic pressure in humans. Rev ADM 2005; 62: 171-176.

  23. Denbesten PK. Effects of fluoride on protein secretion and removal during enamel development in the rat. J Dent Res 1986; 65: 1272-1277.

  24. Denvesten PK. Mechanism and timing of fluoride effects on developing enamel. J Pub Health Dent 1999; 59: 274-251.

  25. Heindel JJ, Bates HK, Price CJ, Marr MC, Myers CB, Schwetz BA. Developmental toxicity evaluation of sodium fluoride administered to rats and rabbits in drinking water. Fund App Toxicol 1996; 30: 162-177.

  26. Nicolau J, Ferreira ML. In vitro and in vivo effects of fluoride solution on human salivary amylase. Fluoride 2001; 34: 55-60.

  27. Hara K, Yu MH. Effect of fluoride on human salivary amylase activity. Fluoride 1995; 28: 71-74.

  28. Shahed AR, Biondi J, Allmann DE. Effect of NaF on rat parotid gland amylase activity and cAMP concentration in vitro and in vivo. J Dent Res 1985;64:1126-1129.

  29. Lowe SW, Ruley HE, Jacks T, Housman DE. p53 dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell 1993; 74: 957-967.

  30. Lane DP. p53, guardian of the genome. Nature 1984; 77: 1087-1090.

  31. Hall PA, Coates PJ, Ansari B, Hopwood D. Regulation of cell number in the mammalian gastrointestinal tract: the importance of apoptosis. J Cell Sci 1994; 107: 3569-3577.

  32. Reed JC. Double identity for proteins off the Bcl-2 family. Nature 1997; 387: 773-776.

  33. Bronner MP, Culin C, Reed JC, Furth EE. The bcl-2 proto-oncogene and the gastrointestinal epithelial tumor progression model. Am J Path 1995; 146: 20-26.

  34. Romero F, Martínez CA, Camonis J, Rebollo A. Aiolos transcription factor controls cell death in T cells by regulating Bcl-2 expression and its cellular localization. EMBO J 1999; 18: 3419-3430.

  35. Parck JR, Hockenbery DM. Bcl-2: a novel regulator of apoptosis. J Cell Biochem 1996; 60: 12-17.

  36. Shashi A. In vivo studies concerning toxic effects of sodium fluoride on hepatic function in rabbits. Fluoride 2003; 36: 30-37.

  37. Guan Z, Xiao K, Zeng X, Long Y, Chen Y, Jiang S. Changed cellular membrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis. Arch Toxicol 2000; 74: 602-608.




2020     |     www.medigraphic.com