2006, Número 3
Aislamiento y caracterización de cepas de Bordetella bronchiseptica de origen canino
Molina GG, Rosales ME, Bárcenas MG, Montaraz CJA
Idioma: Español/Inglés
Referencias bibliográficas: 42
Paginas: 313-325
Archivo PDF: 288.14 Kb.
RESUMEN
Se aislaron, identificaron y caracterizaron cepas de
Bordetella bronchiseptica de origen canino. Con ese propósito se tomaron exudados nasales de perros con semiótica de afecciones respiratorias y clínicamente sanos, con ayuda de hisopos impregnados con medio infusión cerebro-corazón. Éstos se cultivaron en agar Mac Conkey incubando a 37°C durante 48 h; las colonias presuntivas de
Bordetella bronchiseptica se identificaron por movilidad, tinción de Gram y pruebas bioquímicas. Las cepas se caracterizaron por sus factores de virulencia: hemólisis, hemaglutinación, producción de dermonecrotoxina y presencia de pertactina. Se tomaron 130 exudados en un lapso de ocho meses y se lograron 11 aislamientos. Once animales presentaron afecciones en vías respiratorias y se aislaron seis cepas; ninguna de éstas mostró hemólisis franca, los títulos de hemaglutinación variaron entre las diferentes cepas. Todas las cepas, menos una, mostraron actividad de dermonecrotoxina, y sólo cuatro produjeron pertactina.
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