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Revista Latinoamericana de Microbiología

2003, Número 1-2

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Microbiología 2003; 45 (1-2)


El uso de microarreglos para estudiar la patogénesis de Helicobacter pylori

Morales EMR, Delgado SG, Cravioto A

Idioma: Ingles.
Referencias bibliográficas: 45
Paginas: 24-29
Archivo PDF: 74.12 Kb.


RESUMEN

La disponibilidad de las secuencias de los genomas de varios microorganismos, a través de bases de datos y del análisis computacional de las mismas, ha permitido el desarrollo de nuevas áreas tales como: el análisis in silico, la genómica comparativa, la genómica funcional, la transcriptómica, la proteómica y la farmacogenómica. Con la tecnología de microarreglos, se ha logrado analizar, de manera global, la expresión de miles de genes y puede ser aplicada a diversos sistemas biológicos. El uso de esta tecnología y el acceso a las secuencias de los genes que conforman los genomas de dos cepas de H. pylori y del genoma humano, ha permitido a varios grupos de investigación reportar resultados importantes en los últimos años. Estos reportes muestran, por un lado, la gran diversidad de contenido genético en diferentes cepas de H. pylori, la diferente expresión de genes del microorganismo en diferentes condiciones de desarrollo, así como la asociación de la expresión de grupos de genes bacterianos con diferentes patologías gástricas. Por otro lado, analizan la respuesta transcripcional global de la célula epitelial gástrica inducida por la infección con H. pylori, reportando alteración en la expresión de genes relacionados con funciones de transcripción, con señales transduccionales, con la regulación y la diferenciación del ciclo celular, con factores de desarrollo, con la alteración en el balance apoptosis/proliferación, con la expresión de proteínas de membrana y con la respuesta inflamatoria del huésped.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Alm, R.A., L.-S. Ling, D.T. Moir, B.L. King, E.D. Brown, P.C. Doig, D.R. Smith, B. Noonan, B.C. Guild, B.L. deJonge, G. Carmel, P.J. Tummino, A. Caruso, M. Uria.Nickelsen, D.M. Mills, C. Ives, R. Gibson, D. Merberg, S.D. Mills, Q. Jiang, D.E. Taylor, G.F. Vovis & T.J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of human gastric pathogen Helicobacter pylori. Nature 397:176-180.

  2. Allan, E., C.L. Clayton, A. McLaren, D.M. Wallace & B.W. Wren. 2001. Characterization of the low-pH responses of Helicobacter pylori using genomic DNA arrays. Microbiology 147:2285-2292.

  3. Ang, S., C. Lee, K. Peck, M. Sindici, U. Matrubutham, M.A. Gleeson & J.T. Wang. 2001. Acid-induces gene expression in Helicobacter pylori: study in genomic scale by microarray. Infect. Immun. 69:1679-1686.

  4. Aspinall, G.O. & M.A. Monteiro. 1996. Lipopolysaccharides of Helicobacter pylori strains P466 and MO19: structures of the O antigen and core oligosaccharide strains. Biochemistry 35:2498-2504.

  5. Aspinall, G.O., M.A. Monteiro, H. Pang, E.J. Walsh & A.P. Moran. 1994. O antigen chains in the lipopolysaccharide of Helicobacter pylori NCTC 11637. Carbohydr. Lett. 1:151-156.

  6. Atherton, J., P. Cao, R. Peek, M. Tummuru, M. Blaser & T. Cover. 1995. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J. Biol. Chem. 270:17771-17777.

  7. Atherton, J.C. 1997. The clinical relevance of strains types of Helicobacter pylori. Gut 40:701-703.

  8. Björkholm, B.M., J.L. Guruge, J.D. Oh, A.J. Syder, N. Salama, K. Guilemin, S, Falkow, C. Nilsson, P.G. Falk, L. Engstrand & J.I. Gordon. 2002. Colonization of germ-transgenic mice with genotyped Helicobacter pylori strains from a case-control study of gastric cancer reveals a correlation between host responses and HsdS components of type I restriction-modification systems. J. Biol. Chem. 277:34191-34197.

  9. Blaser, M., G. Perez-Perez, H. Kleanthous, T. Cover, R. Peek, P. Chyou, G. Stemmermann & A. Nomura. 1995. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res. 55:2111-2115.

  10. Cecini, S., C. Lange, Z. Xiang, J.E. Cabtree, P. Giara, M. Borodovsky, R. Rappuoli & A. Covacci. 1996. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. USA. 93: 14648-14653.

  11. Covacci, A., S. Censini, M. Bugnoli,. R. Petraca, D. Burroni, G. Macchia, A. Masone, E. Papini, Z. Xiang, N. Figura & R. Rappuoli. 1993. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc. Natl. Acad. Sci. USA. 90:5791-5795.

  12. Cover, T., M. Tummuru, P. Cao, A. Stuart & M. Blaser. 1994. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J. Biol. Chem. 269:10566-10573.

  13. Cover, T.L., W. Puryear, G.I.Perez-Perez & M.J. Blaser. 1991. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infec. Immun. 59:1264-1270.

  14. Cox, J.M., C.L. Clayton, T. Tomita, D.M. Wallace, P.A. Robinson & J.E. Crabtree. 2001. cDNA array analysis of cag pathogenicity island-associated Helicobacter pylori epithelial cell response genes. Infec. Immun. 69:6970-6980.

  15. Chiou, C.-C., C.-C. Chan, D.-L. Sheu, K.-T. Chen, Y.-S. Li & E.-C. Chan. 2001. Helicobacter pylori infection induced alteration of gene expression in human gastric cells. Gut 48:598-604.

  16. DeRisi, J.L., V.R. Iyer & P.O. Brown. 1997. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278:680-686.

  17. Dunn, B., G. Campbell, G. Perez-Perez & M. Blaser. 1990. Purification and characterization of urease from Helicobacter pylori. J. Biol. Chem. 265:9464-9468.

  18. Evans, D., D. Queiroz, E. Mendes & D. Evans Jr. 1998. Helicobacter pylori CagA status and s and m alleles of vacA in isolates from individuals with a variety of H. pylori associated gastric diseases. J. Clin. Microbiol. 36:3435-3437.

  19. Guillemin, K., N.R. Salama, L.S. Tompkins & S. Falkow. 2002. Cag pathogenicity island-specific responses of gastric epithelial cells to Helicobacter pylori infection. Proc. Natl. Acad. Sci. USA. 99:15136-15141.

  20. Han, S.R., H.J. Schreiber, S. Bhakdi, M. Loos & M. Maeurer. 1998. vacA genotypes and genetic diversity in clinical isolates of Helicobacter pylori. Clin. Diagnos. Lab. Immun. 5:139-145.

  21. Harrington, C.A., C. Rosenow & J. Retief. 2000. Monitoring gene expression using DNA microarrays. Curr. Opin. Microbiol. 3:285-291.

  22. Harris, P.R., T.L. Cover, D.R. Crowe, J.M. Orenstein, M.F. Graham, M.J. Blaser & P.D. Smith. 1996. Helicobacter pylori cytotoxin induces vacuolation of primary human mucosal epithelial cell. Infec. Immun. 64:4867-4871.

  23. Hippo, Y., H. Taniguchi, S. Tsutsumi, N. Machida, J.M. Chong, M. Fukayama, T. Kodama & H. Aburatani. 2002. Global gene expression analysis of gastric cancer by oligonucleotide microarrays. Cancer Research. 62:233-240.

  24. Hirata, Y., S. Maeda, Y. Mitsuno M. Akanuma, Y. Yamaji, K. Ogura, H. Yoshida, Y. Shiratori & M. Omata. 2001. Helicobacter pylori activates the cyclin D1 gene through mitogen-activated protein kinase pathway in gastric cancer cells. Infect. Immun. 69:3965-3971.

  25. http://www.nhgri.nih.gov/DIR/LCG/15K/HTML/aboutmicro.html.

  26. Israel, D.A., N. Salama, C.N. Arnold, S.F. Moss, T. Ando, H.-P. Wirth, K.T. Tham, M. Camorlinga, M. Blaser, S. Falkow & R.M. Peek Jr. 2001. Helicobacter pylori strain-specific differences in genetic content, identified by microarray, influence host inflammatory responses. J. Clin. Invest. 107:611-620.

  27. Israel, D.A., N. Salama, U. Krishna, U.M. Rieger, J.C. Atherton, S. Falkow & R.M. Peek Jr. 2001. Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc. Natl. Acad. Sci. USA. 98:14625-14630.

  28. Jungblut, P.R., D. Bumann, G. Haas, U. Zimny-Arndt, P. Holland, S. Lamer, F. Siejak, A. Aebischer & T.F. Meyer. 2000. Comparative proteome analysis of Helicobacter pylori. Mol. Microbiol. 36:710-725.

  29. Kirikoshi, H., H. Sekihara & M. Katoh. 2001. Up-regulation of WNT10A by tumor necrosis factor-alpha and Helicobacter pylori in gastric cancer. Int. J. Oncol. 19:533-536.

  30. Lucchini, S., A. Thompson & J.C.D. Hinton. 2001. Microarrays for microbiologists. Microbiology 147:1403-1414.

  31. Maeda, S., M. Otsuka, Y. Hirata, Y. Mitsuno, H. Yoshida, Y. Shiratori, Y. Masuho, A. Muramatsu, N. Seki & M. Omata. 2001. cDNA microarray analysis of Helicobacter pylori-mediated alteration of gene expression in gastric cancer cells. Biochem. Biophys. Res. Commun. 284:443-449.

  32. Mai, U.E.H., G.I. Perez-Perez, J.B. Allen, S.M. Eahl, M.J. Blaser & P.D. Smith. 1992. Surface proteins from Helicobacter pylori exhibit chemotactic activity for human leukocytes and are present in gastric mucosa. J. Exp. Med. 175:517-525.

  33. Mitsuno, Y., H. Yoshida, S. Maeda K. Ogura, Y. Hirata, T. Kawabe, Y. Shiratori & M. Omata. 2001. Helicobacter pylori induced transactivation of SRE and AP-1 through the ERK signaling pathway in gastric cancer cells. Gut 49:18-22.

  34. Odenbreit, S., J. Püls, B. Sedlmaier, E. Gerland, W. Fischer & R. Haas. 2000. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion. Science 287:1497-1500.

  35. Peek Jr., R., G. Miller, K. Tham, G. Perez-Perez, T. Cover, J. Atherton, G. Dunn & M. Blaser. 1995. Detection of Helicobacter pylori gene expression in human gastric mucosa. J. Clin. Microbiol. 33:28-32.

  36. Perez-Perez G, A. Olivares, T. Cover, M. Blaser. 1992. Characteristic of Helicobacter pylori variants selected for urease defficiency. Infect. Immun. 60:3658-3663.

  37. Rudi, J., C. Kolb, M. Maiwald, D. Kuck, A. Sieg, P. Galle & W. Stremmel. 1998. Diversity of Helicobacter pylori vacA and cagA genes and relationship to VacA and protein expression, cytotoxin production, and associated diseases. J. Clin. Microbiol. 36:944-948.

  38. Salama, N., K. Guillemin, T.K. McDaniel, G. Sherlock, L. Tompkins & S. Falkow. 2000. A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains. Proc. Natl. Acad. Sci. USA. 97:14668-14673.

  39. Schena, M., D. Salon, R.W. Davis & P.O. Brown. 1995. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270:467-470.

  40. Sepulveda, A.R., H. Tao, E. Carloni, J. Sepulveda, D.Y. Graham & L.E. Peterson. 2002. Screening of gene expression profiles in gastric epithelial cells induced by Helicobacter pylori using microarrays analysis. Aliment. Pharmacol. Ther. 16:(suppl. 2)145-157.

  41. Suganuma, M., M. Kurusu, S. Okabe, N. Sueoka, M. Yoshida, Y. Wakatsuki & H. Fujiki. 2001. Helicobacter pylori membrane protein 1: a new carcinogenetic factor of Helicobacter pylori. Cancer Res. 61:6356-6359.

  42. Tomb, J.F., O. White, A.R. Kerlavage, R.A. Clayton, G.G. Sutton, R.D. Fleischmann, K.A. Ketchum, H.P. Klenk, S. Gill, B.A. Dougherty, K. Nelson, J. Quackenbush, L. Zhou, E.F. Kirkness, S. Peterson, B. Loftus, D. Richardson, R. Dodson, H.G. Khalak, A. Glodek, K. McKenney, L.M. Fitzegerald, N. Lee, M.D. Adams, E.K. Hickey, D.E. Berg, J.D. Gocayne, T.R. Utterback, J.D. Peterson, J.M. Kelley, M.D. Cotton, J.M. Weidman, C. Fujii, C. Bowman, L. Watthey, E. Wallin, W.S. Hayes, M. Borodovsky, P.D. Karp, H.O. Smith, C.M. Fraser & J.C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388:539-547.

  43. Velculescu, V.E., L. Zhang, B. Vogelstein & K.W. Kinzler. 1995. Serial analysis of gene expression. Science 270:484-487.

  44. Wanbura, C., N. Aoyama, D. Shirasaka, T. Sakai, T. Ikemura, M. Sakashita, S. Maekawa, K. Kuroda, T. Inoue, S. Ebara, M. Miyamoto & M. Kasuga. 2002. Effect of Helicobacter pylori-induced cyclooxygenase-2 on gastric epithelial cell kinetics: implication for gastric carcinogenesis. Helicobacter 7:129-138.

  45. Whitfield, M.L., G. Sherlock, A.J. Saldanha, J.I. Murray, C.A. Ball, K.E. Alexander, J.C. Matese, C.M. Perou, M.M. Hurt, P.O. Brown & D. Botstein. 2002. Identification of genes periodically expressed in the human cell cycle and their expression in tumors. Mol. Biol. Cell. 13:1977-2000.




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