García-Parra MI, Jiménez-Coello M, Carrillo-Ávila BA, Sauri EEA, Martínez-Aguilar VM, Chávez-Cortéz EG

Idioma: Ingles.
Referencias bibliográficas: 37
Paginas: 147-155
Archivo PDF: 220.01 Kb.

RESUMEN

El propósito de este estudio fue la cuantificación de Esfingosina-1-Fosfato (S1P) en las bolsas periodontales de pacientes con periodontitis. Estudio observacional, descriptivo de casos y controles. 30 sujetos fueron seleccionados de los cuales 15 controles y 15 casos. Se realizó un estudio periodontal completo siguiendo los parámetros establecidos por la AAP en 2017 para el diagnóstico de las enfermedades periodontales. Se tomaron muestras de saliva y de líquido crevicular gingival de cada sujeto estudiado y se analizaron con el ELISA kit humano para S1P (MyBiosource #MBS2516132) y de acuerdo con las instrucciones del fabricante, se realizó para cuantificar la presencia d S1P en las muestras estudiadas. Los resultados mostraron diferencia significativa (p=0.05) entre casos y controles. En el caso de las muestras de saliva, la concentración de S1P en controles fue mayor (72.94 ng/mL y 45.12 ng/mL). Para Líquido crevicular gingival, se encontró mayor cantidad de S1P en los pacientes con periodontitis (20.09 ng/mL y 15.20 ng/mL). Este estudio plantea una posible ruta de metabolismo óseo, proceso inflamatorio e identificación de la Periodontitis a través de la cuantificación oral de S1P, sin embargo se necesitan estudios futuros.

REFERENCIAS (EN ESTE ARTÍCULO)

1. Barbato L., Francioni E., Bianchi M., Mascitelli E., Brancato L., Tonelli D. Periodontitis and bone metabolism. Clin Cases in Miner Bone Metab. 2015; 12 (2): 174-177.

2. Hajishengallis G. Periodontitis: from microbial immune subversion to systemic inflammation. Nat Rev Immunol. 2015; 15 (1): 30-44.

3. Baeza M., Garrido M., Hernandez-Rios P., Dezerega A., Garcıa-Sesnich J., Strauss F., Aitken J.P., Lesaffre E., Vanbelle S., Gamonal J., Brignardello-Petersen R., Tervahartiala T., Sorsa T, Hernandez M. Diagnostic accuracy for apical and chronic periodontitis biomarkers in gingival crevicular fluid: an exploratory study. J Clin Periodontol. 2016; 43 (1): 34-45

4. Bostanci N., Belibasakis G. Gingival crevicular fluid and its immune mediators in the proteomic era. Periodontol 2000. 2018; 76 (1): 68-84.

5. Suchetha A., Shahna N., Sapna N., Darshan B., Apoorva S., Bhat D. Gingival crevicular fluid: A Review of literature. Annals of Dental Specialty. 2018; 6 (2): 211-216.

6. Duvina M., Barbato L., Brancato L., Delle Rose G., Amunni F., Tonelli P. Biochemical markers as predictors of bone remodelling in dental disorders: a narrative description of literature. Clin cases in Miner Bone Metab. 2012; 9 (2): 100-106.

7. Taubman M., Valverde P., Han X., Kawai T. Immune Response: The Key to Bone Resorption in Periodontal Disease. J Periodontol. 2005; 76 (11): 2033-2041.

8. Naruishi K., Nagata T. Biological effects of interleukin 6 on Gingival Fibroblasts: Cytokine regulation in periodontitis. J Cell Physiol. 2018; 233 (9): 6393-400.

9. Wright H., McCarthy H.S., Middleton J., Marshall M. RANK, RANKL and osteoprotegerin in bone biology and disease. Curr Rev Musculoskelet Med. 2009; 2 (1): 56-64.

10. Nagasawa T., Kiji M., Yashiro R., Hormdee D., Lu H., Kunze M., Suda T., Koshy G., Kobayashi H., Oda S., Nitta H., Ishikawa, I. Roles of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin in periodontal health and disease. Periodontol 2000. 2007; 43 (1): 65-84.

11. Kapasa E., Giannoudis P., Jia X., Yang X. The effect of rankl/opg balance on reducing implant complications. J Funct Biomater. 2017; 8 (4): 42:2-10.

12. Reynolds G.M., Visentin B., Sabbadini R. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines. Methods Mol Biol. 2018; 1697: 43-56.

13. Meshcheryakova A., Mechtcheriakova D., Pietschmann P. Sphingosine 1-phosphate signaling in bone remodeling: Multifaceted roles and therapeutic potential. Expert Opin Ther Targets.2017; 21 (7): 725-737.

14. Xiao L., Zhou Y., Friis T., Beagley K. and Xiao Y. S1P-S1PR1 Signaling: the “Sphinx” in Osteoimmunology. Front. Immunol. 2019; 10: 1409:1-18.

15. Ishii M., & Kikuta J. Sphingosine-1- phosphate signaling controlling osteoclasts and bone homeostasis. Biochim Biophys Acta. 2013; 1831 (1): 223-227.

16. Yu H., Sun C., Argraves K. Periodontal inflammation and alveolar bone loss induced by Aggregatibacter actinomycetemcomitans is attenuated in sphingosine kinase 1- deficient mice. J Periodont Res. 2016; 51 (1): 38-49.

17. Ishii M., Egen J.G., Klauschen F., Meier- Schellersheim M., Saeki, Y., Vacher J., Germain R.N. Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis. Nature. 2009; 458 (7237): 524-528.

18. Ishii M., Shimazu Y., Meier-Schellersheim M., Germain R. Chemorepulsion by blood S1P regulates osteoclast precursor mobilization and bone remodeling invivo. J Exp Med. 2010; 207 (13): 2793-2798.

19. Aoki M., Aoki H., Ramanathan R., C. Hait N., Takabe K. Sphingosine-1-Phosphate Signaling in Immune Cells and Inflammation: Roles and Therapeutic Potential. Mediators of Inflamm. 2016; 2016: 1-11.

20. Maceyka M., Harikumar K., Milstien S., Spiegel S. Sphingosine-1-phosphate signaling and its role in disease. Trends Cell Biol. 2012; 22 (1): 50-60.

21. Caton, J., Armitage, G., Berglundh, T., et al. A new classification scheme for periodontal and peri-implant diseases and conditions- Introduction and key changes from the 1999 classification. J Clin Periodontol. 2018; 45 (20): S1-S8.

22. Dame Z., Aziat F., Mandal R., Krishnamurthy R,1Bouatra S, Borzoui S, et al. The Human Saliva Metabolome. Metabolomics. 2015; 11 (1): 864-83.

23. Leppilahti, J.M., Hernández-Ríos, P.A., Gamonal, J.A., Tervahartiala, T., Brignardello-Petersen, R, Mantyla, P, Sorsa, T, Hernández, M. Matrix metalloproteinases and myeloperoxidase in gingival crevicular fluid provide site-specific diagnostic value for chronic periodontitis. J Clin Periodontol. 2014; 41: 348-356.

24. Gamonal J., Bascones A., Jorge O., Silva A. Chemokine RANTES in gingival crevicular fluid of Adult patients with periodontitis. J Clin Periodontol. 2000; 27: 675-681.

25. Navazesh, M. Methods for collecting saliva. Ann Ny Acad Sci. 1993; 694 (1): 72-77.

26. Spring Bio Science. Phosphate Buffered Saline with Tween 20 (20x). California. 2012.

27. World Health Organization. Obesity and overweight. OMS April 2020. URL: https://www.who.int/es/news-room/fact-sheets/detail/obesity-and-overweight. Accessed May 2020.

28. Sartawi Z., Schipani E., Ryan K., Waeber C. Sphingosine 1-phosphate (S1P) signalling: Role in bone biology and potential therapeutic target for bone repair. Pharmacol Res. 2017; 125: 232-245.

29. Hutami I., Tanaka E., Izawa T. Crosstalk between Fas and S1P1 signaling via NF-kB in osteoclasts controls bone destruction in the TMJ due to rheumatoid arthritis. Jpn Dent Sci Rev. 2019: 55 (1):12-19.

30. Obinata H., Hla T. Sphingosine 1-phosphate and inflammation. Int immunol. 2019; 31 (9): 617-625.

31. Proia R., Hla T. Emerging biology of sphingosine-1-phosphate: its role in pathogenesis and therapy. J Clin Invest. 2015; 125 (4): 1379-1387.

32. Xiao Y., Wu J., Yuan Y., Guo X., Chen B., Huang Q. Effect of moesin phosphorylation on high-dose sphingosine-1-phosphate-induced endothelial responses. Mol Med Rep. 2018; 17 (1): 1933-1939.

33. Keller J., Catala-Lehnen P., Huebner A., Jeschke A., Heckt T., Lueth A., Schilling S., et. al. Calcitonin controls bone formation by inhibiting the release of sphingosine 1- phosphate from osteoclasts. Nat Commun. 2014; 5: 5215:1-13.

34. Khavandgar Z., Murshed M. Sphingolipid metabolism and its role in the skeletal tissues. Cell Mol Life Sci. 2015; 72 (5): 959-969.

35. Barembaum S., Azcurra A. La saliva: una potencial herramienta en la Odontología. Rev Fac Odont. 2019; 29 (2): 9-21.

36. Koss M., Castro C., Salúm K., López M. Changes in saliva protein composition in patients with periodontal disease. Acta Odontológica Latinoamericana. 2009; 22 (2): 105-112.

37. Gohda M., Kunisawa J., Miura F., Kagiyama Y., Kurashima Y., Higuchi, M., et al. Sphingosine 1-phosphate regulates the egress of IgA plasmablasts from Peyer’s patches for intestinal IgA responses. J Immunol. 2008; 180 (8): 5335- 5343.